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菌物学报  2011 

Activity changes of several extracellular enzymes in liquid culture of Phellinus mori
桑木层孔菌液体培养过程中几种胞外酶活性的变化

Keywords: amylase,pectinase,carboxymethyl cellulose enzyme,laccase,enzyme activity
淀粉酶,果胶酶,羧甲基纤维素酶,漆酶,酶活

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Abstract:

Phellinus mori had rich extracellular enzymes. The amylase activity in liquid culture of the fungus had the maximum value on day 7. Pectinase showed two peaks on day 9 and 12, respectively. The maximum value of carboxymethyl cellulose enzyme (CMCase) activity appeared on day 10. The laccase reached its peak on day 9. It was indicated that Phellinus mori preferred starch to pectin, cellulose and lignin. The protein concentration had two peaks on day 8 and 11, respectively. The reducing sugar concentration decreased gradually with the incubation time, and the pH value decreased on days 2-7 but increased after day 8.

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