Establishment of ISSR-PCR system and preliminary analysis of genetic diversity for Ampelomyces quisqualis
白粉寄生孢ISSR-PCR体系的建立及遗传多样性的初步分析

The reaction parameters and primers of ISSR-PCR for Ampelomyces quisqualis (Aq) were optimized by an orthogonal design and a single factor test. The genetic diversity of Aq was analyzed by the ISSR-PCR reaction system. Polymorphic bands of 73 isolates generated by five primers indicate that high diversity is conserved in Aq. The dendrogram based on ISSR results shows that the genetic diversity of Aq was relatively associated with the diversity of host fungi and host plants.