Effects of extracts from different phytomedicines on cell growth and intracellular triterpenoids formation of Ganoderma lucidum in submerged fermentation
不同植物药提取物对灵芝细胞生长和胞内三萜产物形成的影响

The effects of water and ethanol extracts from different phytomedicines on mycelial growth and intracellular triterpenoids (ITS) formation of Ganoderma lucidum in submerged fermentation were studied. The medicinal fungus G. lucidum was inoculated into the basal media with and without addition of extract of phytomedicines, and then cultured for 7d. The results showed that both water extracts from Lonicera japonica and Lycium chinensis at 100mg/L increased mycelial biomass production of G. lucidium (p<0.05); while the water extract from Forsythia suspensa had significantly stimulatory effects on both the mycelial growth and ITS production, and the ITS yield increased from (192.54±8.99)mg/L to (302.52±3.79)mg/L when the concentration of the water extract was 400mg/L. In addition, ethanol extracts from L. japonica and L. chinensis at 200mg/L showed positive effects on the mycelial growth, and the ethanol extract from L. chinensis displayed stimulatory effects on ITS production. However, both the water extracts and ethanol extracts from Isatis tinctoria and Ginkgo biloba showed the inhibitory effects on the mycelial growth and ITS production of G. lucidium.