In vitro anti-tumor and antimicrobial activity of acidic triterpenoids from submerged culture of Ganoderma sinense
紫芝酸性三萜类化合物体外抑癌和抑菌作用的研究

The in vitro anti-tumor activity of acidic triterpenoids from Ganoderma sinense mycelia and submerged fermentation liquid by using 3-(4, 5-dimethylthiozol -2-y1)-2, 5-diphenyltetrazolium bromide (MTT) method, as well as the antimicrobial activity on several bacteria and molds by using cylinder-plate method were investigated. The results showed that both the intracellular and extracellular acidic triterpenoids at 250μg/mL displayed significant inhibitory effects on human hepatoma cell line BEL7402 and human breast carcinoma cell line MCF-7 (P < 0.05); however, they had no significant inhibitory effects on human gastric cancer cell Line SGC-7901. The growth of BEL7402 cells treated by intracellular acidic triterpenoids was significantly inhibited, and there was no exponential phase in the growth curve. After 3d culture, BEL7402 cells treated by intracellular acidic triterpenoids appeared fewer and smaller as compared with the cells in control. The acidic triterponoids at 40mg/mL had significantly inhibitory effect on the growth of Escherichia coli and Staphylococcus aureus (P < 0.01), however, the sample had little inhibitory effect on Bacillus subtitis and Penicillium chrysogenum, and had no inhibitory effect on Aspergzillus niger. Further studies revealed that the minimal inhibitory concentration (MIC) of the acidic triterpenoids to E. coil, S. aureus, B. subtitis, A. niger and P. chrysogenum was 20mg/mL, 20mg/mL, 40mg/mL, 80mg/mL and 40mg/mL, respectively. The antibacterial components of the acidic triterpenoids sample couldn't be destroyed in 60℃ treatment for 2h, but it could be partly destroyed in 80 ℃ treatment for 2h, thus the antimicrobial activity was decreased.