Fermentation culture of Naematoloma fasciculare and antitumor activities of its fermented broth in vivo
簇生沿丝伞液体发酵培养及其发酵物体内抗肿瘤活性研究

Optimum conditions of fermentation culture of Naematoloma fasciculare were investigated by using central composite rotatable design. Antitumor activities of fermented broth of N. fasciculare were observed on H22 implanted mice in vivo. The results showed that the maximal biomass was 15.06g/L when the composition of the medium was 59.27g/L maltose, 8.04g/L peptone:yeast powder=1:1 and 2.27g/L KH2PO4:MgSO4:(NH4)2SO4=1:1:1. There was an obvious dose-effect relationship between dosages of fermented broth of N. fasciculare and antitumor rates, and the maximum inhibition rate reached 54.71% at concentration of 200mg/kg. In addition, WBC (109/L) and content of IL-2 of the mice in the group at concentration of 200mg/kg were all improved obviously as compared with control group.