Culture study of Marssonina coronaria from diseased apple leaves
苹果盘二孢Marssonina coronaria的分离培养研究

Marssonina coronaria is the causal agent of Marssonina leaf blotch of apple which leads severe defoliation starting at early summer.It is difficult to obtain the pure culture of this fungus because of its slow growth.Three isolation methods were compared by using 4 culture media in this study.The results showed that each isolation method was successful to obtain the pure culture of fungus.However,single spore isolation was much easier,less contamination and more successful than isolation using diseased leaf...