The methods for rapid DNA extraction from teliospores of Puccinia xanthii f. sp. ambrosiae-trifidae
苍耳柄锈菌三裂叶豚草专化型冬孢子基因组DNA快速提取方法的比较研究

The genomic DNA of Puccinia xanthii f. sp. ambrosiae-trifidae was extracted by nine methods from the teliospores. The results show that the methods of CTAB-steel balls, modified mini-drill and modified EZ-Kit were better than the other six methods due to their higher quality of DNA extraction with less impurity. The template DNA samples generated from these methods were detected by methods of ITS-PCR and ISSR-PCR with the primer UBC#835. They all produced expected band patterns. The research confirms that the DNA samples generated from these three methods could be used for further molecular study of the rust fungus.