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菌物学报 2011
Extraction conditions of glomalin-related soil protein
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Abstract:
Glomalin is a soil glycoprotein produced by arbuscular mycorrhizal (AM) fungi,presently named as glomalin-related soil protein (GRSP) due to its unspecific extraction. The extraction conditions of GRSP can affect its measured value,therefore,the influences of pre-sterilization of soil sample,centrifugation and soil sample mass on the measured value of GRSP were studied with citrus orchard soil. The results showed that both pre-sterilization of soil sample and centrifugation at higher centrifugal force (15,0...