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菌物学报  2011 

Extraction conditions of glomalin-related soil protein
球囊霉素相关土壤蛋白的提取条件

Keywords: arbuscular mycorrhizal fungi,extraction of protein,centrifugal force,sterilization,soil sample mass
丛枝菌根真菌,蛋白提取,离心力,灭菌,土样质量

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Abstract:

Glomalin is a soil glycoprotein produced by arbuscular mycorrhizal (AM) fungi,presently named as glomalin-related soil protein (GRSP) due to its unspecific extraction. The extraction conditions of GRSP can affect its measured value,therefore,the influences of pre-sterilization of soil sample,centrifugation and soil sample mass on the measured value of GRSP were studied with citrus orchard soil. The results showed that both pre-sterilization of soil sample and centrifugation at higher centrifugal force (15,0...

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