INTER-ISOLATE HETEROKARYOSIS IN PAECILOMYCES TENUIPES
细脚拟青霉田间分离菌株间的异核现象

Heterokaryons were synthesized between monoconidial progeny of dif- ferent field isolates of Paecilomyces tenuipes.Heterokaryotic hyphae appeared as a solid line of white mycelial tufts where contact occurred between colonies of compatible isola- tes when they were paired on a modified Sabouraud's agar medium containing yeast extract and maltose.A small mycelial fragment from the tufted zone was transfered to Petri dishes containing water agar and hyphal tip isolates were obtained from the resulting colonies. Thirty or more monoconidial isolates were further obtained from each hyphal tip isolate and plated on PDA in Petri dishes.The resulting cultures were compared with the two paren- tal isolates that produced the tufted reaction.Heterokaryosis was demonstrated when differ- ent monoconidial isolates fro mhyphal tip cultures C_5,B_3,F_3 were representative of both parental types and/or when new colony morphologies were recovered.One of 6 different combinations involving 4 different field isolates produced heterokaryons.Heterokaryons were formed at a frekuency of 13.5% between the monoconidial progeny of isolates 803 and 1401.