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菌物学报  2007 

RAPD analysis of Phaeoporus obliquus strains from different areas of China
应用RAPD标记对斜生褐孔菌菌株亲缘关系的分析

Keywords: Sclerotium,DNA fingerprint,genetic distance
菌核
,DNA指纹图谱,遗传距离

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Abstract:

The relationship of 8 Phaeoporus obliquus strains isolated from sclerotia collected from different areas of China was studied by use of random amplified polymorphic DNA (RAPD) markers. The DNA fingerprints of different strains showed that there were genetic diversity among the strains tested. The results indicated that 12 random primers generated 167 bands in total,of which 101 bands showed polymorphism and the average polymorphism rate was 60.5%. There are a little intraspecific difference among fingerprints of the strains. At the level of 0.508 (genetic distance),BCX01 and BCX02 were clustered in the same group and the second group included JL01, JL02, JL03, JL04 and JL05. HLJ01 was independent. Genetic variability of different strains is related to geographical origins.

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