Application of high-performance liquid chromatographic fingerprinting in the study of tissue isolation of Ganoderma lucidum
HPLC指纹图谱技术在灵芝组织分离试验中的应用

Using high-performance liquid chromatographic fingerprinting, the differences of the strains isolated from different parts of the same fruiting body of Ganoderma lucidum were investigated. Four parts of fruiting bodies were used for tissue isolation and the isolated strains were cultured on four media. The similarities between chromatograms of these samples were calculated. The results are as follows: under the same culture condition, the similarities of HPLC fingerprints of the strains isolated from different parts were all higher than 0.99. Meanwhile, the similarities of HPLC fingerprints of the strains isolated from the same part were all higher than 0.96 in different media. The effects of different isolation tissues and media to the HPLC chromatograms of the fruiting bodies were not distinctive. The triterpenoid content of strain 18 cultured on medium C is the highest among the tested fruiting bodies. The best isolated part is the upper layer of pileus and medium C is the best medium. The triterpenoid composition in the fruiting bodies of Ganoderma lucidum is mainly decided by their genetic factors; the growth condition will affect the content of triterpenoid compound and this effect can be showed by different peak-area ratios of the chromatogram. This is the first report of HPLC fingerprinting method applied to the analysis of tissue isolation of Ganoderma lucidum.