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菌物学报 2008
DNA identification of Tricholoma giganteum isolates
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Abstract:
The total ITS rDNA sequences of fruitbodies of Tricholoma giganteum and Tricholoma matsutake, and the mycelia of cultures isolated from fruitbodies of T. giganteum were obtained and analyzed. The results indicated that the length of entire ITS of T. giganteum was 589bp, and T. matsutake 601bp. The length discrepancy and sequence polymorphism of ITS1 and ITS2 were existed between the two species, but there was little ITS variability of intraspecies in T. giganteum. The mycelia isolated had the same ITS sequences as their original basidiocarps. The results also showed that the germplasm of T. giganteum could be diagnosed by ITS sequences.
