Isolation and identification of sedative and hypnotic compound from the fermentation broth of Hirsutella sinensis
中国被毛孢发酵液中一种镇静催眠活性物质的分离纯化和结构鉴定

The acetylacetate extract of the fermentation broth of Hirsutella sinensis RCEF0273 having sedative and hypnotic activity was analysed. By means of bioactive guided isolation of column chromatography and high performance chromatography, a white pure compound was obtained, and the compound was identified as 9-glucopyranosyl theophylline by the methods of mass spectroscopy (MS) and nuclear magnetic resonance (NMR). Sedative and hypnotic activity of 9-glucopyranosyl theophylline was traced, and the results showed the compound significantly inhibited the spontaneous activity of rats. Moreover, the compound shortened the sleep latency, and prolonged the duration of sleep of rats.