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菌物学报  2008 

Antagonistic effects of Bacillus cereus B-02 on Botrytis cinerea
Bacillus cereus B-02对Botrytis cinerea 拮抗机理的研究

Keywords: electron microscopy,laser scanning confocal microscopy,mycelial cell,ultrastructure
电子显微镜术
,激光扫描共聚焦显微镜术,菌丝细胞,超微结构

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Abstract:

To investigate the antifungal effects of antagonistic bacteria Bacillus cereus B-02 on Botrytis cinerea, the normal and treated mycelial cells were observed by scanning electron microscope (SEM), transmission electron microscope (TEM) and laser scanning confocal microscope (LSCM). The results showed that the configuration of mycelial cells treated with strain B-02 culture supernatant was destroyed and there was a lot of unmembranous material and vacuoles in the cell. Fluorescence intensity of the treated cells and that of the cells of control group was different very significantly. It indicated that the synthesis of DNA, the mitochondrial membrane potential and reactive oxygen standard of Botrytis cinerea hyphae were greatly influenced by B. cereus B-02 culture supernatant.

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