TAGGING THE GENES INVOLVED IN TRAPPING-DEVICE IN NEMATODE-TRAPPING FUNGI II. THE MORPHOLOGY AND SOUTHERN BLOT ANALYSIS OF THE TRANSFORMANT NOT FORMING TRAPPING DEVICE
捕食线虫真菌捕食器相关基因标记II.捕食器基因不表达转化子的形态特征及核酸杂交分析

Totally 2135 transformants were generated after transforming the nematode- trapping fungus Arthrobotrys oligopsora with plasmid pBChygro. Among these transformants, one (YMF1.00110) losing the ability to form trapping-device was screened. This transformant grows slowly and shows different morphologies as compared with the wild type. The results of Southern blot analysis indicated the transfromant had single copy integrations of pBChygro. Furthermore, the integration pattern and tagged efficiency of parts of transformants also were presented in this paper.