Studies on Tissue Culture of Streptocarpus wendlanddii
旋果苣组织培养的研究

Leaf, scape and bud of Streptocarpus wendlanddii were used as explants in this study. Based on MS medium, the appropriate approach and sterilization time of tissue culture and rapid propagation of Streptocarpus wendlanddii were obtained via regulating the concentrations of cytokinin and auxin. The results showed that when the explants were sterilized in 1 g/L mercuric chloride, the optimum sterilization time for leaf was 4-5 minutes, while bud was 4 minutes. When the explants were sterilized with 84 disinfectant liquid, the suitable time for scape and bud was 5 minutes. The optimal medium for callus induction was MS+BA 1.0 mg/L+NAA 1.0 mg/L;the optimal medium for bud differentiation was MS+BA 4.0 mg/L+NAA 0.2 mg/L;the optimal medium for root differentiation was MS+BA 1.0 mg/L+NAA 1.0 mg/L;the optimal medium for subculture was MS+BA 1.0 mg/L+KT 0.5 mg/L.