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菌物学报  2003 

GENETICS OF ENZYMES RELATED TO THE PATHWAY OF NITRATE REDUCTASE IN FUSARIUM MONILIFORME
串珠镰孢霉硝酸盐还原途径酶的遗传研究*

Keywords: Nitrate reduction pathway,nit-mutant,sexual cross,nuclear gene,cytogene
硝酸盐还原途径
,nit突变体,有性杂交,细胞核基因,细胞质基因

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Abstract:

Studies on the genetic relationship between molybdenum-containing cofactor and nitrite reductase in Fusarium moniliforme were carried out by sexual cross. The results of sexual cross between molybdenum-containing cofactor mutants (nitB) and nitrite reductase mutants (nitC) showed that there were two set of genes to control the enzymes in different mating populations and in the same mating population of the pathogen but on different hosts. It could be hypothesized that nuclear gene and cytogene might co-control the enzymes related to nitrate utilization pathway. If the ratio of segregation is 1:1:1:1 to four different phenotypes (nitD, nitB, nitC, wt), it means that molybdenum-containing cofactor and nitrite reductase were controlled by one set of nuclear gene, when the progenies had only two phenotypes, the male parent phenotype was disappeared, and the ratio of female parent phenotype and wild type was 1:1, indicating that besides nuclear gene, cytogene might be involved in controlling the enzymes.

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