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OALib Journal期刊
ISSN: 2333-9721
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Induction of Hairy Roots of Panax quinquefolium by Ri Plasmid and Research on Suitable Culture Condition of Panax quinquefolium Hairy Roots
西洋参发根的诱导及其培养条件的研究

Keywords: bioengineering,hairy root,Agrobacterium rhizogenes,Panax quinquefolium L
生物工程
,发根,农杆菌,西洋参

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Abstract:

Hairy roots of Panax quinquefolium L.were induced from the root explants of two-year old Panax quinquefolium L.by Agrobacterium rhizogenes A4 with direct inoculation,the cultured clones of the hairy roots were established on solid 1/2 MS and MS mediums.After several times of subcultures,the hairy roots still maintained vigorous growth.After the determination of UV-spectrophotometry,the total ginsenosides content in the hairy root came up to 3.88%.The suitable culture conditions for Panax quinquefolium L.hai...

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