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吉林农业大学学报 2010
Induction of Hairy Roots of Panax quinquefolium by Ri Plasmid and Research on Suitable Culture Condition of Panax quinquefolium Hairy Roots
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Abstract:
Hairy roots of Panax quinquefolium L.were induced from the root explants of two-year old Panax quinquefolium L.by Agrobacterium rhizogenes A4 with direct inoculation,the cultured clones of the hairy roots were established on solid 1/2 MS and MS mediums.After several times of subcultures,the hairy roots still maintained vigorous growth.After the determination of UV-spectrophotometry,the total ginsenosides content in the hairy root came up to 3.88%.The suitable culture conditions for Panax quinquefolium L.hai...