Cloning and Sequence Analysis of Gene ORFⅠ of Strawberry vein banding virus(SVBV)
草莓镶脉病毒(SVBV)ORFⅠ基因的克隆及序列分析

The total DNA was extracted from strawberry leaves infected with SVBV by CTAB method.Specific primer pair was designed to amplify the fragment including SVBV ORFⅠ,and then the fragment was cloned and sequenced.Sequence analysis results showed that the fragment was compzosed of 1 033 nts,including 987 nts of full-length ORFⅠgene encoding 328 amino acids.Comparing the sequence of SVBV ORFⅠwith that of the ORFⅠof other members of Caulimovirus,the result showed that Chinese SVBV ORFⅠshared the highest sequence similarity(88.3%) with that of the American SVBV.A phylogenetic tree based on alignment of the nucleotide sequences of ORF Ⅰof SVBV and other members of Caulimovirus was constructed.The result indicated that the Chinese SVBV ORFⅠand the American SVBV ORFⅠclustered into a separate subgroup.It illustrated that the two SVBV derived from strawberry had the closest relationship.