Development of SCAR markers based on SRAP and ISSR for rapid identification of Ganoderma strains
利用SRAP和ISSR建立快速鉴定灵芝属菌株的SCAR标记

Forty-eight DNA pools were prepared using standardized DNA extracted from 152 Ganoderma isolates (128 Chinese isolates and 24 non-Chinese isolates) delineated on the basis of ERIC-PCR data. SRAP and ISSR methodologies were used to amplify DNA from the different pools, and four specific marker bands (one SRAP and three ISSR) were selected, cloned and sequenced. After conversion into more stable and more highly specific SCAR markers, a multiplex PCR system was developed and optimized using the corresponding SCAR primers. The feasibility and reliability of adopting strain-specific SCAR markers for the rapid identification of Ganoderma strains were confirmed.