ESTABLISHMENT OF A TRANSFORMATION SYSTEM OF GANODERMA LUCIDUM USING PEG METHOD
利用PEG法建立药用真菌灵芝的转化系统

A procedure of transforming foreign genes into Ganoderma lucidum using PEG buffer has been established. The transformation efficiency of this method reached to 5-6 transformants/mg plasmid 107 protoplast.Transformants could still show HmB resistance after cultivating over 5 subcultures on media without HmB. An analysis of Ganoderma lucidum transformants using Southern blot revealed that foreign gene had integrated into Ganoderma lucidums genome.This study has laid a foundation for those applications, such as improving medical quality of Ganoderma lucidum by gene engineering and producing foreign protein with important economic value by fermentation of Ganoderma lucidum.It is also helpful for us to understand mechanism of gene expresstion and regulation about Ganoderma lucidum.