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菌物学报 2008
Nuclear matrix of Physarum compressum phanero-plasmodium
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Abstract:
Nuclear matrix prepared from phaneroplasmodium of Physarum compressum was observed under TEM. The nuclear matrix with compound-fibres was obtained by use of 2mol/L NaCl + TritonX-100/NP40, while nuclear matrix with nuclear lamina was obtained by using Lis + TritonX-100/NP40. Net nuclear matrix was obtained without using RNase, showing RNA played an important role in nuclear matrix structure. The significance of dissociated nuclei of plasmodium in the study of cell biology was expounded in this paper.