ISOLATION, PURIFICATION AND STRUCTURAL COMPARISON INVESTIGATION OF WATER SOLUBLE POLYSACCHARIDE GFb OF FERMENTED MYCELIA FROM GANODERMA TSUGAE
松杉灵芝发酵菌丝体中水溶多糖的分离纯化与结构的比较研究

The water soluble polysaccharide GFb was isolated and purified from fermented mycelia of Ganoderma isugae. Its homogeneity was comfirmed by chroma-tography on Sepharose CL-4B and glass fiber electrophoresis. Its molecular weight estimated by gel filtration is 98000. By means of IR, GC, GC-MS, 13C-NMR, periodate oxidation, Smith degradation,, methylation analysis and partial hydrolysis, etc., the structure of GFb was identified. Its basic structure is composed of (1-6)-linked galactose and (1-6)-linked glucose residues in main chain, and (1-)-linked galactose, (1-3)-linked and (1-4)-linked glucose residues in side chains. Side chains attached to 0-3 of glucose and galactose residues in main chain. The rate of branch points is 50 percent in main chain and branch rate is 55.6 percent in molecules.