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菌物学报 2003
INTERACTION BETWEEN ROOT AND CALLUS OF PINUS DENSIFLORA AND AN ECTOMYCORRHIZAL FUNGUS, TRICHOLOMA MA TSUTAKE
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Abstract:
A simple in vitro system is described for interaction between tissues of Pinus densiflora and an ectomycorrhiza fungus, Tricholoma matustake. The callus and roots, which were initiated from hypocotyl segments, were inoculated with T. matsutake in artificial condition. After two-week incubation, fungal hyphae had colonized the root surface. Fungal hyphae occurred within the root cortex, and Hartig net was formed after three-week. A few fungal hyphae and Hartig net-like structures were also visible within the callus after three-week, while they were rather less abundant in callus as compared with roots.