GENETIC TRANSFORMATION OF EXSEROHILUM TURCICUM MEDIATED BY AGROBACTERIUM TUMEFACIENS
农杆菌介导的玉米大斑病菌转化

A transformation technique for Exserohilum turcicum mediated by Agrobacterium tumefaciens using vegetative mycelium as recipient was developed and procedure for the enhancement of transformation efficiency was optimized. The binary vector used in transformation was pUR5750, which contains selection marker gene conferring hygromycin-resistance under control of the Aspergillus nidulans gpd promoter and trpC terminator between the left border and right border repeats of the binary vector pBIN19. PCR analysis showed that T-DNA was integrated into the genome of E. turcicum.