THE RELATIONSHIPS BETWEEN DNA POLYMORPHISM ANDGEOGRAPHICAL ORIGIN OF AUSTRALIAN ISOLATES OF VERTICILLIUM DAHLIAE FROM COTTON PLANTS
澳大利亚棉花黄萎病菌DNA多态性及其地理分布相关性研究

Ninety-nine isolates of Verticillium dahliae collected from cotton Plants in eight majorproduction regions in South--Eastern Australia were assessed for DNA polymorphism using theRAPD--PCR tecnnique. A Total of 92 RAPD bands were amplified using 10 random decamer primers,and 55.4% were found to be polymorphic. Variations in RAPD banding patterns detected for these V.dahliae isolates reveal that there is significant genetic diversity between populations of thisphytopathogen, with cluster analysis of the RAPD bands resulting in the classification of 15 RAPDgrouch (RGs). Ten of these RGs could subsequently be classified into three clades which correlatedstrongly with the locality of fungal isolation, and members of the remaining five RGs, however,showed no such correlation with place of origin.