DIFFERENTIATION BETWEEN THE TWO PHENOTYPICALLY SIMILAR YEAST SPECIES CANDIDA MALTOSA AND CANDIDA TROPICALIS BY ELECTROPHORETIC KARYOTYPING
表型相似的热带假丝酵母和麦芽糖假丝酵母在脉冲电泳核型上的差异

The two hydrocarbon-utilizing yeast species Candida maltosa Komagata, Nakase & Katsuya and Candida tropicalis (Castellani) Berkhout are not easy to be clearly distinguished from each other by conventional methods because of their similar morphological, physiological and biochemical characters. Electrophoretic karyotypes of the strains preserved in the China General Microbiological Culture Collection Center (CGMCC) under these two species were analyzed by using pulsed field gel electrophoresis (PFGE). The type and representative strains of C. maltosa were clearly differentiated from those of C. tropicalis in chromosomal DNA banding patterns, while all the strains studied within each of these two species showed similar or identical electrophoretic karyotypes. The difference in electrophoretic karyotypes between C maltosa and C tropiclais coincided well with their different abilities to assimilate soluble starch and to grow at 40 C.