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菌物学报  2008 

Experimental studies on the safety of Pythium guiyangense X.Q. Su to six species of cultivated plants common in Guizhou Province
灭蚊真菌-贵阳腐霉对植物的安全性试验

Keywords: Oomycetes,soil inoculation method,caudex injection method,foliage spray method
灭蚊真菌
,植物,安全性试验,实验方法

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Abstract:

The aim of this study is to investigate whether Pythium guiyangense, a mosquito-killing fungus isolated in Guiyang, Guizhou Province of China in 1994, is pathogenic to plants. Six common crops, Cucumis sativa, Lycopersicon esculentum, Capsicum annuum, Nicotiana tabacum, Brassica campestris and Oryza sativa were used as subjects for test. Zoospores of the fungus were used to infect the plants with soil inoculation method, caudex injection method and foliage spray method. Both positive control (using P. aphanidermatum) and negative control (using sterile water) were set up in all the experiments. The results showed that no infection was found on the tested plants in soil inoculation experiments. In caudex injection test, callus grew around the wounded tissue in most of the plants. Brownish rottenness could be found only in the injected wounds in a few plants, probably caused by saprophytic bacteria or other fungi, and the germ-carrying plants grew normally. No abnormal appearance was found on the six crops in foliage spray test. It was demonstrated that P. guiyangense could hardly infect plants in nature, and was a safe and promising agent for mosquito biological control.

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