COMPARISON OF STAINING EFFECTS OF URANINE AND COOMASSIE BRILLIANT BLUE R-250 ON BLUMERIA GRAMINIS F.SP. TRITICI
荧光素钠和考马斯亮蓝应用于小麦白粉病菌染色效果比较*

Staining effects of uranine and Coomassie brilliant blue R-250 on Blumeria graminis f.sp. tritici was observed. The results showed that uranine staining need only 20 min. of sample treatment, without inhibiting pathogen growth. Uranine was mainly accumulated in the septum and cytoplasm of living pathogen, maintaining brilliant green fluorescence for about 7 min. By use of fluorescence microscopy, the development of the pathogen on the wheat leaves surface could be detected and the dead and alive pathogen could be distinguished. Coomassie brilliant blue R-250 took about 40 min. for treating sample, and the host cell was turned into light blue, and pathogen dark blue. This is a rapid staining method for observing infection structures of B. graminis f.sp. tritici on wheat epidermis or in infected cell, such as primary germtube, appressorial germtube, mature appressoria, pristine haustorium and secondary finger-like haustoria as well as conidia.