Multiple effects of Se and La on morphological and quantitative characters of Agaricus brasiliensis fruit bodies
硒镧复合作用对巴西蘑菇子实体形态和数量特征的影响

The multiple effects of Se and La on morphological and quantitative characters of Agaricus brasiliensis fruit bodies were studied by cultivation of the fungus on medium mixed with different concentration of solutions of Na2SeO3 and LaCl3 and electron microscopy technology. The results showed that there was a very significant increase in sporulation under the treatment of low concentration of Na2SeO3 21.905mg/kg + LaCl3 17.667mg/kg. The number of spores increased with the increase of concentration of the mixed solution and significantly decreased after reaching the peak. The number of cystidia significantly reduced when the fungus grew in the medium with low concentrations (Na2SeO3 21.905mg/kg+LaCl3 17.667mg/kg) and high concentration (Na2SeO3 109.524mg/kg+LaCl3 88.340mg/kg) of Se and La, but the number of cystidia significantly increased under the moderate concentration (Na2SeO3 65.714mg/kg+LaCl3 53.002mg/kg). It was found that the combined application of Se and La affected the length and width of spore, the diameters of cystidia and the width of mycelium. Scanning electron microscopy showed that the spores were shrinking and collapsing, and the mycelium walls were ramified and nodulated under combined application of Na2SeO3 65.714mg/kg+LaCl3 53.002mg/kg. Furthermore the surface ornamentation of cystidia became fuzzy and disappearing, and some cystidia were disappearing and collapsing.