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菌物学报 1998
MORPHOLOGICAL STUDIES ON THE PROCESS OF NUCLEAR RECONSTRUCTION AFTER MITOSIS IN PHYSARUM POLYCEPHALUM
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Abstract:
The process of nuclear morphological construchon after mitosis inPhysarum polycephalum was studied by conventionally stained and "En bloc"silver-stained electron microscopic techniques. Prenucleoar substance of forming newnucleolus was distributed in the outer part of central chromosomes region in metaphasenucleus, and aboved at the two poles together with chromosomes in telophase. Thenucleolar substance was mixed with chromatin when daughter nucleus was formed,andthen the nucleolar substance united with each other and gradually separated fromchromatin,and finally formed a big nucleolus. Chromatin began to decondense inanaphase and further decondense in the new formed nucleus and formed loosechromatin thread structure at minute 60 in interphase. Chromatin began t0 recondenseafter decondensation and formed some condensed chromatin lumps. With thedevelopment of nucleus, many chromatin lumps with different size and shape wereformed betWeen the nuclear membrane and nucleolus.