CLONING OF AN ENDOCHITINASE GENE FROM TRICHODERMA VIRIDE AND ITS TRANSFORMATION INTO CHAETOMIUM GLOBOSUM
绿色木霉内切几丁质酶基因的克隆及其毛壳菌转化*

An endochitinase encoding gene was cloned from the genome DNA of Trichoderma viride. The length of this DNA fragment is 1672 bp and contains a promoter and coding region for mRNA. After ligating it with the promoter and terminator of Trp synthetase from Aspergillus nidulan, this gene was introduced into Chaetomium globosum CG10. It was indicated about one third transformants increased their endochitinase activity significantly. This experiment lays some foundation and present a potential way for improvement of biocontrol activity of Chaetomium globosum with genetic engineering method.