PREPARATION,REGENERATION AND FUSION OF THE PROTOPLAST OF GANODERMA LUCIDUM
灵芝原生质体制备、再生及融合的研究

This paper studied the output and regeneration and fusion of theprotoplastS of strains mH1 and mK1 of Ganoderma lucidum. The results showed thatthe output of the protoplastS was different under different age of hyphae, enzymeconsistency and stabliser. For strain mH1, its optimal age of hyphe, enzymeconsistency and stabliser was 48hr, 3% and 0.6mol / L sucrose respechvely, the strainmK1 was 40hr, 1% and 0.4mol / L mannitol. The frequency of regenerahon of thetwo strains were the best and 3.20 X 10-2 (strains mH1) and 5.40 X 10-2 (strain mKI)sep~ly when the stabliser was 0.6mol / L sucrose. Fusion experimentS betWeen theabove tWo strains were done. The frequency of fusion, after treating with 30% PEG,was 1 .40 X 10 -2.