FLOW CYTOMETRIC ANALYSIS OF TOTAL DNA PER CELL IN CANDIDA SPECIES
几种念珠菌DNA总含量的流式细胞分析

The total DNA per cell of eight Candida isolates in stationary-phase cultures were determined by flow cytometry, in which seven species were included, two strains of Candida albicans, one strain of C. tropicalis, C. krusei, C, parapsilosis, C. kefyr, C. stellatoidea (C. albicans serotype 5) and C. guilliermo ndii. One step staining of EB(ethidum bromide) was used to stain the cells and CRBC as internal parameter standard to assay total DNA per cell. Histograms of stationary-growth-phase Candida isolates demonstrate that the majority of popula. tion is in the G0/G1 phase of the DNA synthetic cycle. Inter-and Intra-specific differences in total DNA per cell were verified statistically by U test.