A Fast Auto-focusing Algorithm of Two-Layer Objects in Microscopy
显微镜下两层物体的快速自动对焦算法

Aiming at the problem of large-scale microscopy images acquisition of two-layer object,an auto-focusing algorithm was proposed based on image edge and adjacent area forecasting.Using the edge area as focusing window and linear forecasting method,the system can acquire clear image of upper object with little interfererence of the background.The experimental results demonstrate that the proposed algorithm is superior in focusing speed,precision and capability of resisting background interference.