Isolation and Identification of degrading bacteria in diesel contaminated soil and study on their degradation capability
油污土中降解柴油细菌的分离鉴定及降解能力研究

The biodegradation bacteria were enriched and isolated from the diesel contaminated soil. Two dominant strains were obtained and named as B-3, B-4. These strains were identified as Tetrathiobacter kashmirensis and Pseudomonas sp. through morphological observation and 16S rDNA sequence analysis, respectively. The degradation capabilities of strain B-3 and strain B-4 were studied in this paper. The strains B-3 and B-4 were taken in the culture medium, in which diesel concentration was 3.78 g/L. The degradation rate of them was 60.98% and 37.18%, respectively. And the results of morphological observation and growth curve measurement to strain B-3 showed that the degradation capability is obviously higher than B-4. To our knowledge, this is also the newly established species about biodegrading diesel contaminated soil using strain B-3.