Comparison of DGGE, T-RFLP and LH-PCR in the analysis of microbial community diversity for two kinds of activated sludges
DGGE、T-RFLP 、LH-PCR对两种活性污泥的微生物种群多样性分析的比较

Using three PCR-based molecular ecological techniques,i.e.DGGE,T-RFLP and LH-PCR,the microbial community diversity of activated sludges from two typical wastewater treatment systems(domestic and coking) was investigated.The advantages and disadvantages of the three techniques were compared and the consideration of method selection under different conditions was suggested.DGGE displayed many bands,nevertheless,the sources of error were multiple.T-RFLP was sensitive,but it required suitable endonuclease and r...