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环境工程学报 2010
Isolation of biosurfactant producing microorganisms and influence on degradation of diesel oil
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Abstract:
Seven biosurfactant producing strains, obtained from sewage of a restaurant by method of microbe cultivation, were respectively interacted with a diesel oil degradable bacteria (a kind of Pseudomonas strains obtained also in lab) to study the influences of biosurfactants on diesel oil degradation. The interaction showed that B26, one of the seven strains, could not only reduce the degradation induction peoriod from 6 days to 4 days but also increase the degradation rate from 71.1% to 80.6%, which deserved further research. All tests were focused on analysis of mechanism and the affecting factors in diesel oil degradation. Based on the morphological, physiological and biochemical test results, B26 was identified as Bacillus sp., in whose metabolism lipopeptide biosurfactant could be produced, and 1.57 g/L of them could be extracted using the freeze-drying method.