Application of PCR-ARDRA technology in screening and identifiying oil degrading strains
PCR-酶切技术在石油烃降解菌筛选鉴定中的应用

In this study,pure culture microbial strains,which were acclimated by crude oil,were used as templates.A way of screening and identifying of oil-degrading bacteria was established,using PCR technique(premier 8f,1492r) and ARDRA patterns which used enzyme digestion(RsaI and MspI) of cloned 16S rDNA gene sequences.The results showed that 60 strains could be divided into 11 operational axonomic unit(OTU).And the ecological characteristics of these cloning communities could be analyzed by this method.The experi...