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ISSN: 2333-9721
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Effects of salinity on activities of H+-ATPase, H+-PPase and membrane lipid composition in plasma membrane and tonoplast vesicles isolated from soybean (Glycine max L.) seedlings

Keywords: salinity,Glycine max L,plasma membrane,tonoplast,H,-ATPase,H,-PPase,membrane lipid composition
盐分效应
,氯化钠,H^+-腺苷三磷酸酶,液泡膜,大豆播种,油脂成分

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Abstract:

The effects of NaCl stress on the H -ATPase, H -PPase activity and lipid composition of plasma membrane(PM) and tonoplast(TP) vesicles isolated from roots and leaves of two soybean cultivars(Glycine max L.) differing in salt tolerance(Wenfeng7, salt-tolerant; Union, salt-sensitive) were investigated. When Wenfeng7 was treated with 0.3%(W/V) NaCl for 3 d, the H -ATPase activities in PM and TP from roots and leaves exhibited a reduction and an enhancement, respectively. The H -PPase activity in TP from roots also increased. Similar effects were not observed in roots of Union. In addition, the increases of phospholipid content and ratios of phospholipid to galactolipid in PM and TP from roots and leaves of Wenfeng7 may also change membrane permeability and hence affect salt tolerance.

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