Investigation of protein-styrene oxide adducts as a molecular biomarker of human exposed to styrene
Investigation of protein-styrene oxide adducts as a molecularbiomarker of human exposed to styrene

Hemoglobin styrene oxide adducts in blood have been studied as a molecular biomarker of worker exposed to styrene. Determination of protein styrene oxide adducts in different biological samples with modified Raney Ni procedure is described in this paper. The following biological samples have been investigated: fresh rat blood reacted with styrene oxide in vitro; rat blood reacted with styrene or styrene oxide in vivo; vein blood from workers exposed to styrene in two factories. The data showed that there was a good linear dose response relationship between reacting dose of styrene oxide or styrene and amount of protein styrene oxide adducts in both in vitro and in vivo experiments. For human samples, a dose response relationship between protein adducts and styrene exposure can be found in glass fiber factory, but not in piano manufacture plant.