Research on preparation,antioxidant function and immumodulatory function of degraded lambda-carrageenan
λ-卡拉胶降解组分的分离纯化、抗氧化及免疫活性研究

Three kinds of polysaccharides(CF1, CF2, CF3) were prepared by anion-exchange column chromatography from degraded lambda-carrageenan PC2 which was obtained by microwave technique. The effects of CF1, CF2 and CF3 on clearing hydroxyl radical, superoxide anion radical and H>2O2 have been studied on the paper. The results showed that all three principles had scavenging effects on radical and could advance the immunity of healthy mice. The intensities of the scavenging effects were relative to the molecular weight and sulfute contents. The lower the molecular weight of lambda-carrageenan was, the stronger the immumodulatory was.The higher the sulfute contents of lambda-carrageenan was, the stronger the intensity of scavenging was. The IC50 values of CF1, CF2 and CF3 on clearing hydroxyl radical, superoxide anion radical and H2O2 were 0.217, 0.619, 0.087 mg/mL; 0.166, 0.214, 0.090 mg/mL; 0.602, 1.060,0.261 mg/mL separately.