Toxicity and immunogenicity of LPS from pathogenic Vibrio alginolyticus to grouper, Epinephelus malabaricus
致病溶藻弧菌脂多糖对点带石斑鱼毒性和免疫原性的影响

In order to study the toxicity and immunogenicity of the LPS from pathogenic Vibrio alginolyticus to Epinephelus malabaricus, the crude LPS was extracted by hot phenol-water extraction, and was intraperitoneally injected to healthy groupers at different concentrations. It was found that crude LPSs from both the pathogenic strain and the weak pathogenic strain of V. alginolyticus were highly toxic to groupers. The immunogenicity of the crude LPS from V. alginolyticus to the groupers was enhanced at elevated concentrations of LPS. The immunogenicity of high purity LPS from E.coli to the groupers was better than the crude LPS from V. alginolyticus.