Haematococcus pluvialis culture in photobioreactor
利用光生物反应器培养雨生红球藻的研究初探

The green microalga Haematococcus pluvialis was cultured in photobioreactor and light incubator, respectively, to compare the effect on cell growth and astaxanthin accumulation. The effects of pH, dissolved oxygen (DO), and effects of different inoculating densities were also studied. The results indicated that only one day was needed for algae in photobioreactor to enter exponential growth phase and only four days were required to reach the maximum of astaxanthin content during stress stage. The culture period of algae in photobioreactor was shorter than that in light incubator. Slight alkaline condition (pH =7.75 ±0.10) was beneficial to the cell growth. DO increased up to 80% during nutrient culture stage and decreased rapidly during stress stage with a minimum of less than 6%. Since the higher cell inoculum density (2.3×104 cell/mL) showed shorter nutrient culture period (7 days) and different cell inoculum densities had no effect on the length of stress period (only 4 days were needed), we suggested that higher cell inoculum density might be adopted for reducing costs and shorten cycles of algae production.