Cloning and analysis of the phosphoenolpyruvate carboxylase gene of Porphyra haitanensis (Rhodophyta)
坛紫菜磷酸烯醇式丙酮酸羧化酶基因的克隆与序列分析

Phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) plays an important role in photosynthesis. To get a deeper insight into the PEPC gene of Porphyra haitanensis T. J. Chang et B. F. Zheng (Bangiales, Rhodophyta), we cloned a partial-length cDNA by homology cloning and RACE. This sequence includes 2538bp encoding a propetide of 846 amino acid residues. The deduced polypeptide shows high identities with the PEPC genes ranging from bacteria and unicellular algae to green plant. Phylogenetic tree analysis shows that he PEPC gene from the Porphyra haitanensis is a C3-like PEPC and more closely related to the ancestral type.