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海洋科学  2012 

Effect of crude oil on the activity of antioxidant enzyme of scallop Chlamys farreri
原油污染对栉孔扇贝抗氧化酶活性的影响

Keywords: water soluble fraction (WSF) of crude oil,Chlamys farreri,activity of enzyme,Super Oxide Dismutase (SOD),Catalase (CAT)
原油水溶性组分
,栉孔扇贝(Chlamysfarreri),酶活性,超氧化物歧化酶,过氧化氢酶

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Abstract:

The toxic effects of water soluble fraction (WSF) of crude oil on scallop Chlamys farreri were examined through exposure tests at different concentrations. The activities of antioxidases in gill and digestive glands were examined. The results showed that the activities of both SOD (Superoxide Dismutase) and CAT (Catalase) in gills and digestive glands showed a decrease-increase-decrease trend with increased exposure time. Time for the enzymes to reach their highest activities varied with the concentration of WSF. The activity of SOD in digestive gland was induced by 0.08 mg/L of WSF on the 1st day of exposure, followed by inhibitory effect. The digestive gland enzyme activities were inhibited first and then increased with time at 0.21 mg/L and 0.88 mg/L of WSF. At the 4th day of the test, the activities of SOD in gills gland were inhibited by both 0.08 and 0.88mg/L of WSF but induced by 0.21 mg/L of WSF. Our study indicated that the SOD activity could be used as a stress indicator of Chlamys farreri when exposed to petroleum polluted environments.

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