DNA extraction and DGGE analysis of the bacterial community of a deep-sea sediment sample collected at the East Pacific Ocean
东太平洋深海沉积物中DNA的提取及细菌多样性初步分析

The total genomic DNA of a deep-sea sediment sample collected at the East Pacific Ocean was extracted using a combined chemical and protease-lysis method.The size of DNA was about 21 kb,and could be used directly for PCR-based molecular research after purification.PCR-DGGE profile of bacterial 16S rDNA V3 fragments gave rise to more than 15 bands.Blast analysis revealed that most of the retrieved sequences from DGGE bands were closely related to uncultured clones from various marine sediment environments,an...