Extraction of YC-2 from Sinonovacula constricta and its antioxidant activity
缢蛏YC-2 蛋白的提取及抗氧化作用研究

Protein YC-2 was isolated from Sinonovacula constricta by water extraction, ammonia sulphate precipitation (80% saturation), ion exchange chromatography, and gel chromatography, and concentrated by freeze drying. SDS-PAGE and the thiobarbituric acid (TBA) method were used to identify various components and their antioxidant activities in Sinonovacula constricta extracxts. We found that YC-2, a dimeric proteins with a 61 ku large subunit and a 45 ku small subunit, had a high oxidation resistance, the removal rate on ? OH being 99.7 % at 0.627 g/L.