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海洋科学 2009
Genomic DNA isolation and RAPD analysis on Moerella iridescens
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Abstract:
Studies of DNA extraction and RAPD(Random Amplified Polymorphic DNA) analysis were performed on the cultured population of Moerella iridescens in Wenling,Zhejiang Province.Among 11 individuals,six screened from thirty-eight primers,which were reproducible,amplified a total of 247 fragments ranging from 300 bp to 1 600 bp.43 loci were detected and the polymorphic loci(P) were 74.42%.Genetic similarity index(S) among individuals were 0.691 and the average genetic distance(D) were 0.309.The result indicates th...