Effect of temperature and pH on the activity of digestive enzymes in Haliotis diversicolor Reeve
温度和pH对杂色鲍消化酶活力的影响

The effects of reaction temperature and pH on the activities of 4 main digestive enzymes(pepsin,trypsin,amylase,cellulase) from hepatopancreas and digestive tract of Haliotis diversicolor Reeve were studied using enzymology analysis method.The results showed that the digestive enzyme activities of H.diversicolor Reeve were all first increased and then decreased with increased temperature or pH in the setted temperature and pH range.The optimum temperature of pepsin in hepatopancreas and digestive tract was both 50℃,the optimum pH range was 2.6~3.0 and 2.6~3.4 respectively.The optimum temperature of trypsin in hepatopancreas was 40℃ and the optimum pH was 8.6~9.0.The optimum temperature of trypsin in digestive tract was 50℃,and the optimum pH was 8.2.The optimum temperature of amylase in hepatopancreas and digestive tract was 40℃ and 30 ℃ respectively,and the optimum pH was 6.8 and 7.2 respectively.The optimum temperature of cellulase in hepatopancreas and digestive tract was 40~50℃ and 40℃ respectively,and the optimum pH was 5.2 and 4.8 respectively.The activities of amylase and cellulase in hepatopancreas are much stronger than those in digestive tract under the optimum temperature and pH range and have organic specificity,while there is no significant difference in the activities of pepsin and tryptase between hepatopancreas and digestive tract.The activities of different digestive enzymes are also significantly different in the same organ.